LIFA (Lambert Instrument Fluorescence Attachment) for FLIM

FLIM is the abbreviation for "Fluorescence Lifetime Imaging Microscopy" and is the technique that maps the spatial distribution of lifetimes within microscopic images of fixed as well as of living cells.

The LIFA (Lambert Instruments FLIM Attachment) for lifetime imaging microscopy is a dedicated system that allows image acquisition and generation of lifetime images in a matter of seconds. It can be attached to any widefield fluorescence microscope and works in the homodyne frequency domain.

LIFA includes :
- a modulated light source (LEDs or Laser diodes)
- a modulated image intensifier
- a CCD camera
- an integrated Control unit / Signal generator / Power supply
- LI FLIM software

The system is easy to install (within 1 hour) and to operate and does not require an optical bench with laser. The LIFA system has been judged "easy and highly quantitative" for FLIM FRET studies.

LIFA can be combined with :

- Laser TIRF ( Total Internal Reflection Fluorescence)
- Yokogawa spinning disk
- Spectrograph (Spectrally Resolved FLIM)

References for LIFA are available on request.

Some specifications:

Lifetime Range and Resolution	0-300 ns ± 40 ps
Measurement time	1 second typical
Speed	12 lifetime images per second
Modulation frequency	100 MHz max
Available LED wavelengths	365 nm, 405, 448, 468, 489, 538, 630, etc...
Detection ranges	200-600 nm, 450-850 nm, 450-900 nm

          Applications

   - FRET (Foerster Resonant
          Energy Transfer)

   - Probing Microenvironment

   - Oxygen Imaging

   - Medical diagnosis

   - Ion Imaging (pH)

LIFA brochure

LIFA
is manufactured
by