index LIFAPrinciple

info@axiomoptics.com (617) 401-2198

at Imagine Optic Inc.

1 Broadway, 14th floor,

Cambridge, MA 02142, USA

Ph: (617) 401-2198 Fax: (425) 930-9818

info@axiomoptics.com

-FRET (Forster Resonant Energy Transfer)
-Molecular interactions
-Oxygen concentration imaging in cells and tissue
-Microenvironment parameters such as PH
-Biosensors
-Protein conformation
-NADH / FAD fluorescence dynamics
-Viscosity imaging
-Membrane dynamics
-Membrane trafficking

LIFA

is manufactured

by

The frequency domain approach uses a modulated illumination and observation and only needs a few acquired images (minimum 3) to produce a lifetime image.

A modulated, excitation light (LEDs or Laser) is sent to the fluorophore, which will absorb the light and emit fluorescent light at the same modulation frequency, however with a time delay (or phase) and a decreased modulation directly depending on its lifetime.

To measure this phase delay (or modulation decrease), a set of images of the fluorophore is then captured by an intensified camera, modulated at the same frequency, each acquired image corresponding to an arbitrary phase delay introduced on purpose on the camera (for example 12 images taken every 30 degrees).

The LI-FLIM software computes a sinus fit on every pixel of the image, compares this function to a reference sample (for example fluorescein), deduces the actual phase delay, or modulation decrease caused by the fluorophore, and mathematically derives the lifetime. This analysis can be performed on fluorophores exhibiting a single or multi-exponential behavior.