The LIFA Toggel FLIM Microscope system is a next-generation fluorescence lifetime imaging camera (FLIM) that makes functional imaging easier by combining the excellent light sensitivity with image acquisition and data analysis using LIFA software. It minimizes the duration of lifetime measurements, compatible with any wide-field microscope. In addition to its broad range of applicability and advanced technology, Toggel is easy to install and maintain.
The Toggel FLIM Microscope camera features a unique image sensor was designed and optimized especially for fluorescence lifetime imaging applications. This image sensor enables lifetime imaging at unprecedented frame rates with the single-image fluorescence lifetime imaging microscopy (siFLIM) method . The camera records two images during a single exposure that are shifted 180° in phase with respect to each other. Demodulation of fluorescence light and the phase shift introduce a difference in light intensity between two images which can be used to track the changes in the fluorescence lifetime. As a result of recording images simultaneously, the artifacts due to cellular movement are eliminated and photon efficiency of the image acquisition is significantly improved.
The built-in time-lapse feature is a useful tool for tracking the changes in the fluorescence lifetime. This feature is widely used in live-cell imaging. The feature is very easy to use. It only requires the user to set the duration and time between measurements and the software generates the time-lapse video.
The LIFA Toggel FLIM Microscope system provides high throughput screening especially for automated lifetime-based screening and characterization of fluorescent proteins. Thus, it could be a very useful tool for the experiments in which the fluorescence lifetime is an important parameter.
LIFA software guides you through your FLIM experiments from start to finish. All Lambert Instruments hardware is integrated seamlessly so you can focus on your experiment. The software takes care of recording the FLIM data and instantly calculates the fluorescence lifetime.
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