HiCAM Fluo | High-Speed Fluorescence Camera
Sensor technology
Intensified CMOS
Resolution
1280 x 1024 / 1920 x 1080
Pixel pitch
6.6 µm x 6.6 µm / 10 µm x 10 µm
Sensor mode / Frame rate
Global shutter / Up to 2,000 fps
Readout noise
N/A
Interface
CoaXPress
HiCAM FLUO: High Speed Fluorescence Imaging at 1 KHz and Above
The HiCAM FLUO is designed specifically for high-speed fluorescence applications, capable of recording high-resolution images at up to 2,000 fps even in challenging low-light conditions. This is achieved through a cooled image intensifier, ensuring clear and detailed imaging.
Housed in a compact aluminum enclosure, the HiCAM FLUO is easy to attach to any fluorescence microscope, making it a versatile tool for your imaging needs.
Fiber-optically coupled intensifier
The HiCAM FLUO is a gated intensified high-speed camera. It features an integrated fiber-optically coupled image intensifier, combining high-speed imaging with enhanced light sensitivity down to the single photon level. This allows the HiCAM FLUO to operate effectively in low-light conditions without the need for high-intensity light sources, making it ideal for fluorescence imaging.
Cooled image intensifier
The fanless design of the HiCAM minimizes vibrations to ensure sharp images. Very low noise levels are achieved by Peltier cooling the image intensifier. Compared to uncooled intensified cameras, noise levels are reduced by a factor of up to 100 times.
Up to 2,000 fps @ 1920 x 1080 pixels & global shutter
The HiCAM Fluo captures full-resolution images at up to 2,000 fps. By reducing the image resolution and using a smaller portion of the sensor, the camera can achieve even higher frame rates. The HiCAM Fluo’s sensor has a full resolution of 1920 x 1080 pixels and utilizes an electronic global shutter, ensuring all pixels are read simultaneously to avoid rolling shutter effects. Combined with intensifier gating, the exposure time can be shortened to 3 ns.
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| Model | HiCAM Fluo 1,000 fps | HiCAM Fluo 2,000 fps |
|---|---|---|
| Resolution/Speed | 1280 x 1024@ 1050 fps 1200 x 720@ 1500 fps 640 x 480 @ 4000 fps 256 x 256 @ 7500 fps | Full resolution @ 2000 fps 1280 x 960 @ 2500 fps 640 x 480 @ 5100 fps 512 x 384 @ 6400 fps |
| Input Effective Area | 12.78 x 10.2 mm | 19.2 x 10.8 mm |
| Effective Pixel Size | 10 x 10 microns | 10 x 10 microns |
| Frame Rate at Max. Resolution | 1050 | 2000 |
| Spectral Response | Gen 3 GaAsP ( 50 % QE at 550 nm) or GaAs | Gen 3 GaAsP ( 50 % QE at 550 nm) or GaAs |
| Intensifier Type | Single stage | Single stage |
| Bit Depth | 12 | 8 |
| Gating | Down to 40 ns | Down to 40 ns |
| Photocathode Cooling | Yes | Yes |
| Interface | CoaXPress | CoaXPress |
Applications of the HiCAM Fluo include:
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High-speed fluorescence, bioluminescence and chemiluminescence detection for in-vivo imaging
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Time-resolved imaging of fluids for microfluidic research
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Flow visualization and velocity measurements using Particle Image Velocimetry (PIV)
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Time-resolved imaging and spectroscopy using ultra-short exposures
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Laser-Induced Fluorescence (LIF)
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Super-slow motion combustion research for the automotive industry
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Time-resolved imaging in plasma physics research
-
Single-photon imaging for astronomy
Publications featuring HiCAM
Capture Software
Capture is an innovative software designed to control and acquire recordings from up to nine streaming intensified high-speed cameras (Intensifier + Streaming Camera + Frame Grabber). Compatible with popular S series Vision Research streaming cameras and frame grabbers from Silicon Software, Euresys, and Pleora, Capture offers seamless integration and functionality.
With Capture, you can:
- Trigger Recordings Precisely: Use Trigger Offset to start recording at the exact right moment.
- Trim Recordings: Easily edit your footage to keep only what you need.
- Export in Various Formats: Save your videos in multiple widely-supported file formats.
Capture makes the process of intensified high-speed imaging straightforward and efficient, accommodating both single and multi-camera setups.

Options
- Cooled Photocathode for reduced noise
- Photocathodes (S20 UV, S20, S25, GaAs, GaAsP)
- Gating speed (40 or 2 ns)
- On-Board memory (8 or 16 GB)
- SDK or Labview Driver
HiCAM Accessories
- Recording station/computer ( 64 Gb of RAM , 4 TB of SSD drive, 10 TB Hard drive)
- Capture Software for high speed recording
- Choice of lenses
- F or C-Mount adapters
- Coax frame grabbers + SDK




