Description
Specifications
QE Curve
Applications
Videos
Downloads
Publications
ORCA-Fusion & ORCA-Fusion BT sCMOS cameras, in a nutshell
The ORCA-Fusion and ORCA-Fusion BT Hamamatsu cameras are meticulously engineered to optimize camera features, providing high-quality images and reliable data across various lighting levels, particularly in challenging low-light scenarios. Representing the pinnacle of scientific CMOS (sCMOS) technology, these Hamamatsu cameras offer unparalleled specifications, including ultra-low readout noise, uniformity comparable to CCDs, fast frame rates, and high quantum efficiency (QE). The ORCA-Fusion BT utilizes back-thinned sensor technology to achieve nearly perfect sensitivity (95%) at its peak.
However, the true value of the ORCA-Fusion and ORCA-Fusion BT lies in their ability to harness exceptional photon detection and collection capabilities. They enable users to detect even the faintest signals, produce visually striking images with high signal-to-noise ratio (SNR) using minimal photons, capture previously undetectable temporal events, and execute computational analyses with confidence. These Hamamatsu cameras simplify complex experiments and make previously impossible endeavors attainable.
ORCA-Fusion & ORCA-Fusion BT sCMOS cameras, in more details
High-speed readout
The introduction of the ORCA-Fusion family has enabled users to stream 5-megapixel images to their computers at a rate of 89 frames per second. To ensure optimal performance with this high data rate, users can adhere to the guidelines outlined in the PC Recommendations document, accessible under Downloads > Manuals & User Guides.
Lightsheet Readout Mode [PATENTED]
Lightsheet Readout Mode is a unique and patented feature of Hamamatsu sCMOS cameras designed specifically to enhance signal-to-noise ratios in Lightsheet microscopy. For more information about the principle and features of Lightsheet Readout Mode, please visit Hamamatsu blog.
ORCA-Fusion BT advantages vs other BT CMOS cameras
- Extremely low readout noise
- High QE across a wide range of wavelengths
- CCD-like uniformity, minimal high readout noise pixels
- High dynamic range
- 3 readout speeds
- Lightsheet readout mode
- 5.3 Megapixels
- Both USB 3.0 and high speed CoaXPress interfaces.
ORCA-Fusion BT advantages vs EM-CCD cameras
- Resolution
- Binning versatility
- No amplification or excess noise
- High dynamic range
- Large field of view
ORCA-Fusion
ORCA-Fusion BT
Hamamatsu Camera Line Up Catalog
ORCA-Fusion Applications:
- 3D Imaging
- High-speed Ca2+ Imaging
- Multi-dimensional Imaging
- Ratio imaging
- FRET & TIRF & Super-resolution microscopy
- Time lapse fluorescence imaging
- Micromorphological observation
- Real-time confocal microscopy
- Fluorescence in situ hybridization (FISH)
- X-ray scintillator readout
- Wide-field fluorescence microscopy
- Time-lapse imaging
- Spinning disk confocal microscopy
- Lightsheet microscopy
- Super resolution precision localization
- Genetically encoded voltage imaging
- Optogenetics
- Single molecule tracking
- Computational imaging
- Quantum computing
Publications with ORCA-Fusion BT:
- Optogenetic relaxation of actomyosin contractility uncovers mechanistic roles of cortical tension during cytokinesis. Kei Yamamoto, Haruko Miura, Motohiko Ishida, Yusuke Mii, Noriyuki Kinoshita, Shinji Takada, Naoto Ueno, Satoshi Sawai, Yohei Kondo & Kazuhiro Aoki. Nature Communications. December 2021.
- Space-time vector light sheets. Mbaye Diouf, Mitchell Harling, Murat Yessenov, Layton A. Hall, Ayman F. Abouraddy & Kimani C. Toussaint. Optics Express. October 2021.
- Simple-to-use CRISPR-SpCas9/SaCas9/AsCas12a vector series for genome editing in Saccharomyces cerevisiae. Satoshi Okada, Goro Doi, Shitomi Nakagawa, Emiko Kusumoto & Takashi Ito. G3 Genes Genomes Genetics. August 2021.
- Endogenous β-neurexins on axons and within synapses show regulated dynamic behavior. Oliver Klatt, Daniele Repetto, Johannes Brockhaus, Carsten Reissner, Abderazzaq El khallouqi, Astrid Rohlmann, Martin Heine & Markus Missler. Cell Reports. June 2021.
- Single-molecule tracking of chromatin-associated proteins in the C. elegans gonad. Lexy von Diezmann & Ofer Rog. American Chemical Society. June 2021.
- Brain endothelial cell TRPA1 channels initiate neurovascular coupling. Pratish Thakore, Michael G. Alvarado, Sher Ali, Amreen Mughal, Paulo W. Pires, Evan Yamasaki, Harry A.T. Pritchard, Brant E. Isakson, Cam Ha T. Tran & Scott Earley. eLife Sciences. February 2021.
Publications with ORCA-Fusion:
- A synthetic tubular molecular transport system. Pierre Stömmer, Henrik Kiefer, Enzo Kopperger, Maximilian N. Honemann, Massimo Kube, Friedrich C. Simmel, Roland R. Netz & Hendrik Dietz. Nature Communications. July 2021
- An early cell shape transition drives evolutionary expansion of the human forebrain. Silvia Benito-Kwiecinski, Stefano L. Giandomenico, Magdalena Sutcliffe, Erlend S. Riis, Paula Freire-Pritchett, Iva Kelava, Stephanie Wunderlich, Ulrich Martin, Gregory A. Wray, Kate McDole & Madeline A. Lancaster. Cell. April 2021.
- Spatiotemporal single-cell RNA sequencing of developing chicken hearts identifies interplay between cellular differentiation and morphogenesis. Madhav Mantri, Gaetano J. Scuderi, Roozbeh Abedini-Nassab, Michael F. Z. Wang, David McKellar, Hao Shi, Benjamin Grodner, Jonathan T. Butcher & Iwijn De Vlaminck. Nature Communications. March 2021.
- N6-methyladenosine modification of MALAT1 promotes metastasis via reshaping nuclear speckles. Xinyu Wang, Chong Liu, Siwei Zhang, Huiwen Yan, Liwen Zhang, Amin Jiang, Yong Liu, Yun Feng, Di Li, Yuting Guo, Xinyao Hu, Yajing Lin, Pengcheng Bu & Dong Li. Developmental Cell. March 2021.
- Omnitemporal choreographies of all five STIM/Orai and IP3Rs underlie the complexity of mammalian Ca2+ signaling. Scott M. Emrich, Ryan E. Yoast, Ping Xin, Vikas Arige, Larry E. Wagner, Nadine Hempel, Donald L. Gill, James Sneyd, David I. Yule, Mohamed Trebak. Cell Reports. March 2021.
- Three-dimensional single molecule localization close to the coverslip: a comparison of methods exploiting supercritical angle fluorescence. Philipp Zelger, Lisa Bodner, Martin Offterdinger, Lukas Velas, Gerhard J. Schütz & Alexander Jesacher. Biomedical Optics Express. February 2021.
- Volumetric live cell imaging with three-dimensional parallelized RESOLFT microscopy. Andreas Bodén, Francesca Pennacchietti, Giovanna Coceano, Martina Damenti, Michael Ratz & Ilaria Testa. Nature Biotechnology. January 2021.
- A quantitative live-cell superresolution imaging framework for measuring the mobility of single molecules at sites of virus assembly. Nicholas S. Groves, Merissa M. Bruns & Schuyler B. van Engelenburg. Pathogens. November 2020.
- Dual-view oblique plane microscopy (dOPM). Hugh Sparks, Lucas Dent, Chris Bakal, Axel Behrens, Guillaume Salbreux & Chris Dunsby. Biomedical Optics Express. November 2020.
- An optogenetic system to control membrane phospholipid asymmetry through flippase activation in budding yeast. Tomomi Suzuki, Tetsuo Mioka, Kazuma Tanaka & Akira Nagatani. Scientific Reports. July 2020.
- Pooled CRISPR screens with imaging on microraft arrays reveals stress granule-regulatory factors. Emily C. Wheeler, Anthony Q. Vu, Jaclyn M. Einstein, Matthew DiSalvo, Noorsher Ahmed, Eric L. Van Nostrand, Alexander A. Shishkin, Wenhao Jin, Nancy L. Allbritton & Gene W. Yeo. Nature Methods. May 2020.
- Population imaging of neural activity in awake behaving mice. Kiryl D. Piatkevich, Seth Bensussen, Hua-an Tseng, Sanaya N. Shroff, Violeta Gisselle Lopez-Huerta, Demian Park, Erica E. Jung, Or A. Shemesh, Christoph Straub, Howard J. Gritton, Michael F. Romano, Emma Costa, Bernardo L. Sabatini, Zhanyan Fu, Edward S. Boyden & Xue Han. Nature. October 2019.
HCImage Live GUI:
HCImage Live software is included with the ORCA-Fusion & ORCA-Fusion BT cameras and is supported under Windows 10, 8.1 & 7 (both 32-bit & 64-bit) OS. Software user guides can be download under the “Downloads” tab. More information regarding HCImage Live GUI software can be obtained at https://hcimage.com/hcimage-overview/hcimage-live/ .
Other Software Compatibility:
The ORCA-Fusion & ORCA-Fusion BT cameras can also be operated through Micro-Manager.
Options for ORCA-Fusion:
- To operate the camera at full speed it is required to use compatible CoaXPress frame grabber and cables which can be ordered along with the camera.
- This model can also be ordered to be compatible with Nikon F-mount lenses.
Options for ORCA-Fusion BT:
- To operate the camera at full speed it is required to use compatible CoaXPress frame grabber and cables which can be ordered along with the camera.
- This model can also be ordered to be compatible with Nikon F-mount lenses.