Prime BSI & Prime BSI Express | Large Field of View sCMOS Cameras
Sensor Technology
sCMOS
Quantum Efficiency
95%
Readout Noise
1.0 e-
Resolution
6.5 µm x 6.5 µm pixels
Speed
63 FPS (at 11-bit)
Interface
PCle, USB 3.0
Large Field of View sCMOS
The Prime BSI & BSI Express Scientific CMOS (sCMOS) cameras offer an ideal combination of high-resolution imaging and sensitivity due to their optimized pixel design and exceptional 95% Quantum Efficiency.
Compact in size at 78mm x 78mm x 90mm, the Prime BSI Express features optimized cooling, making it perfectly suited for seamless integration into both new and existing configurations.
Key Features
Sensitivity
Achieve a near perfect 95% quantum efficiency with the Prime BSI back-illuminated sCMOS camera. Its innovative design brings light in from the back of the sensor, enabling photons to directly hit the light-receiving surface, thus maximizing light collection capability. With its combination of 95% quantum efficiency and minimal 1.0 e- read noise, the Prime BSI sets a new standard for sensitivity among sCMOS cameras, achieving speeds of over 40 frames per second.

Resolution and Pixel Size
The Prime BSI features 6.5 μm pixels, which is the accepted standard for most live cell applications using 40x and 60x magnification. This pixel size captures highly detailed images across the imaging plane, making it the most suitable for the broadest range of microscope objectives.
Superior Background Quality
The Prime BSI features Pattern Noise Reduction Technology and Correlated Noise Reduction Technology, ensuring clean, pattern free images with minimal pixel defects, resulting in improved image quality in low light conditions.
Advanced Triggering
For applications that need control over the line time, Programmable Scan Mode provides increased control over the rolling shutter exposure and read-out functionality of CMOS sensors.
Sequenced Multiple Acquisition Real Time Streaming (SMART) allows the Prime BSI to capture a continuous sequence of images while cycling through 16 pre-programmed exposure time values. This capability ensures the fastest multi-channel acquisition times possible.

Advanced Features
The Prime BSI features PrimeEnhance active denoising which provides a real-time quantitative increase in the signal to noise ratio to maximize sensitivity at ultra-low light levels.

1
2
3
4
5
6
7
8
| Sensor Specifications | ||
|---|---|---|
| Camera Model | Prime BSI | Prime BSI Express |
| Sensor Brand & Model | Gpixel GSENSE2020BSI | Gpixel GSENSE2020BSI |
| Sensor Technology | sCMOS | sCMOS |
| Sensor Resolution | 2048 x 2048 / 4.2 MP | 2048 x 2048 / 4.2 MP |
| Sensor Size | 13.3 x 13.3 mm² | 13.3 x 13.3 mm² |
| Pixel Size | 6.5 x 6.5 µm² | 6.5 x 6.5 µm² |
| Sensor Shutter | Rolling Shutter | Rolling Shutter |
| Quantum Efficiency (@ 560 nm) | 95% | 95% |
1
2
3
4
5
6
7
8
9
10
| Readout Specifications (Prime BSI & Prime BSI Express) | |||
|---|---|---|---|
| Readout Mode | Combined Gain | CMS | High Gain |
| Bit Depth | 16-bit | 12-bit | 11-bit |
| Readout Noise (rms) | 1.8 e- | 1.1 e- | 1.8 e- |
| Full Well Capacity (typ.) | 45,000 e- | 1,000 e- | 10,000 e- |
| Dynamic Range | 88.0 dB / 25,000:1 | 59.2 dB / 900:1 | 74.9 dB / 5,600:1 |
1
2
3
4
5
6
7
8
| Cooling Specifications | |||
|---|---|---|---|
| Camera Model | Prime BSI | Prime BSI | Prime BSI Express |
| Cooling Method | Air | Water | Air |
| Cooling Temperature | -20°C | -30°C | 0°C |
| Dark Current | 0.5 e-/pixel/s | 0.12 e-/pixel/s | 1.5 e-/pixel/s |
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
| Temporal & Frame Rate Specifications | ||||
|---|---|---|---|---|
| Camera Model | Prime BSI | Prime BSI | Prime BSI Express | Prime BSI Express |
| Readout Mode | Combined Gain / CMS | High-Gain | Combined Gain / CMS | High-Gain |
| Full Frame (2048 x 2048) | 43 fps | 63 fps | 43 fps | 95 fps |
| 1024 x 1024 | 87 fps | 125 fps | 87 fps | 188 fps |
| 512 x 512 | 173 fps | 250 fps | 174 fps | 375 fps |
| 256 x 256 | 346 fps | 497 fps | 347 fps | 745 fps |
| 128 x 128 | 687 fps | 979 fps | 690 fps | 1468 fps |
| Exposure (min.) | 11.4 ms | 10.3 ms | 11.4 ms | - |
1
2
3
4
5
6
7
| Mechanical, Electrical & Environment Specifications | ||
|---|---|---|
| Camera Model | Prime BSI | Prime BSI Express |
| Digital Interface | USB 3.0 / PCIe | USB 3.2 |
| Lens Interface | C-mount | C-mount |
| Dimensions (L x W x H, mm) | 162 x 108 x 108 | 92 x 78 x 78 |
| Weight | 1.7 kg | 1.7 kg |
| AC Adapter Power Supply | 100-240 VAC / 50-60 Hz | 100-240 VAC / 50-60 Hz |
| Camera Power Supply | 12 VDC | 12 VDC |

Prime BSI Applications
- TIRF Microscopy
- Ratiometric Imaging
- Cell Motility
- Light Sheet Microscopy


