Quantitative Phase Imaging
We offer high resolution Quantitative Phase Imaging (QPI) cameras made by Silios, a well know manufacturer of micro-optics and imaging solutions. Quantitative phase imaging (QPI) is a microscopy technique that provides a way to visualize and accurately measure the phase shift of light as it passes through a sample. This is particularly valuable for studying transparent or semi-transparent objects, like biological cells, which are often difficult to see with traditional transmitted light microscopy. Here’s a breakdown of QPI key aspects:
- Phase Shift Measurement:
- QPI focuses on measuring how much the phase of a light wave changes as it interacts with a sample. This change is related to the sample’s optical properties, such as its thickness and refractive index.
- Label-Free Imaging:
- A significant advantage of QPI is that it doesn’t require staining or labeling the sample. This is crucial for studying live cells and dynamic processes without disrupting their natural state.
- Quantitative Information:
- Unlike traditional phase contrast microscopy, QPI provides quantitative data. This means it can measure precise values related to the sample’s properties, like its dry mass, thickness, and refractive index distribution.
- Applications:
- Biological Imaging: QPI is widely used in biology to study cell morphology, dynamics, and other properties.
- Materials Science: It’s also applied in materials science to characterize the structure and properties of materials at the microscopic and nanoscale levels.
- Nanotechnology: QPI assists in visualizing and analyzing nanoscale structures and phenomena.
In essence, QPI allows scientists to gain detailed, quantitative insights into the structure and properties of transparent objects by measuring how they affect the phase of light.